Novel approach to enhance aggregate migration-driven epigenetic memory which induces cardiomyogenic differentiation on a dendrimer-immobilized surface

The dynamic migratory behavior of human mesenchymal stem cells (hMSCs) has a significant impact on the epigenetic profiles that determine fate choice and lineage commitment during differentiation. Here we report novel approach to enhance repeated migration-driven memory which induces cardiomyogenic differentiation dendrimer surface with fifth generation (G5). Cells exhibited formation cell aggregates G5 through active migration morphological changes, these showed strong expression cardiac-specific marker cardiac troponin T (cTnT) at 10 days. When were passaged onto fresh over three passages 40 days, levels multiple markers including GATA4, NKX2.5, MYH7, TNNT2 higher compared those as single cells. To investigate whether hMSCs was enhanced by aggregate memory, reseeded using aggregate-based cell-based passage methods. Analyses global changes in H3 histone modifications pattern increased H3K9ac H3K27me3, decreased H3K9me3 cultures passages. However, their modification PS after initialization reformation cultures. Thus, repetitive led greater degree modification, well gene suggestive